The Effects of Tramadol Hydrochloride Administration on the Hematological and Biochemical Profiles of domestic male Rabbits

Nehad R Elyazji, Ismail Abdel-Aziz, Ayoub Aldalou, Osama Shahwan


The present work was conducted to assess the Hematological and biochemical toxicity profiles of tramadol during therapeutic use. Male adult domestic rabbits (weighting 1000-1300g) were divided into six groups. Group one received vehicle (saline), group two, three and four injected intramuscularly with doses of tramadol equal to 40 mg / kg body weight / day for 10, 20 and 30 days respectively followed by 10 days recovery period for group three and four. Hematological and Biochemical measurements were carried out every 10 days. Control and treated rabbits were sacrificed at the end of 10, 20, 30, and 40 days respectively. Blood was collected in dry centrifuge tubes. Sera were separated and kept at -20?C until analysis. Administration of tramadol at a dose of 40mg/kg. body weight for 30 days increased serum glucose, cholesterol, triglycerides, electrolytes (Na+ and Ca2+) and cholinesterase values. Theses increments were 21.53%, 9.64%, 3.39%, 2.14%, 6.67 and 20% respectively, compared to control level. Also there was a significant increase in kidney function indices. The effect was more pronounced on creatinine. Activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and bilirubin were increased following tramadol injection to rabbits for 30 days. Concerning hematological parameters, the more obvious changes were observed in the increment of white blood cells (WBCs), lymphocyte and MCV value and the decrease in hematocrit, hemoglobin(Hb), red blood cells (RBCs) count, MCH, MCHC, and platelets (PLT) count in response to the administration of tramadol. In general recovery periods after tramadol abstinence, showed signs of improvement of blood indices. Conclusion: Tramadol toxic effects should be avoided during long term therapy specially in large doses. Key words: Tramadol, Gaza Strip, rabbits, blood indices, enzymes. ?


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