Iprodione and a mixture of Carbendazim and Diethofencarb fungicides resistance in Botrytis cinerea population collected from Palestinian vegetable greenhouses

Radwan M Barakat , Mohammad I Al-Masri

Abstract


The acquired resistance of Botrytis cinerea isolates, the causal agent of grey mould disease to synthetic fungicides Iprodione (Rovral®) and a mixture of Carbendazim and Diethofencarb (Resec®) was evaluated on 84 isolates collected from vegetable greenhouses throughout the West Bank. The fungicide-resistant isolates were evaluated in vitro in terms of mycelial growth rate and conidial germination, in vivo as grey mould lesion growth rate on detached bean leaves, and on grey mould disease severity on bean whole plants. The effective concentrations (EC50) of the fungicides varied in vitro and in vivo. The Iprodione EC50 (µg ml-1) on the isolates in vitro (mycelial growth rate and conidial germination) were 0.8 and 4.31 respectively, and in vivo (grey mould lesion growth rate and disease severity) were 16.7 and 336.4, respectively. However, the Carbendazim and Diethofencarb EC50 (µg ml-1) on the isolates in vitro were 1.19 and 10.43 respectively, and in vivo (grey mould lesion growth rate and disease severity) were 16.4 and 447.2, respectively. The Iprodione resistance was found in 49.4% of the isolates; Carbendazim and Diethofencarb resistance on the other hand was evident in 44.4% of the isolates, and resistance for both fungicides occurred in 35.7% of the isolates. The highest percentages of resistance were found in the Northern agricultural areas (Jenin, Nablus, Tulkarem and Qalqelia greenhouses) cultivated traditionally with cucumbers and tomatoes. Keywords: fungicide resistance, Iprodione, Carbendazim and Diethofencarb, Botrytis cinerea.

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