ALTERATIONS IN BODY WEIGHT, PROTEIN PROFILE, NON-PROTEIN NITROGEN CONSTITUENTS AND KIDNEY STRUCTURE IN DIABETIC RATS UNDER GLIBENCLAMIDE TREATMENT

Maged M. Yassin, Abdel Rahiem A. Ashour, Nehad R. Elyazji

Abstract


The effect of glibenclamide administration to alloxan diabetic rats on body weight, protein profile and non-protein nitrogen constituents has been assessed. Histological changes of kidney were also studied. Diabetic and glibenclamide-treated animals showed significant decrease in body weight gain from controls recording percentage values of 36.37% and 21.14%, respectively throughout the study. Highly significant decrease in serum total protein and albumin levels was recorded in diabetic rats throughout the study with percentage decrease of 21.74% and 20.19%, respectively as compared to control levels. This decrease is rendered to be not significant on glibenclamide treatment showing percentage decrease of 3.30% and 7.75%, respectively. The estimated levels of serum globulin in diabetic rats showed significant decrease with a percentage of 24.17% compared to controls. However, non-significant flactuations in globulin levels were achieved upon glibenclamide administration during the whole experimental period with percentage increase of 5.0% as compared to controls. A highly significant increase in serum urea concentration of diabetic animals has been observed. The highest increment reached was at the eighth week where urea recorded 97.33 mg/dl with a percentage increase of 154.99% as compared with control group. Glibenclamide did not manage to retain urea to normal level. Uric acid concentration was generally decreased in diabetic group and this decrease reached a significant value during the last two weeks of the study. Also, creatinine concentration was decreased significantly in diabetic animals recording a magnitude of 37.04% compared to control. However, glibenclamide administration managed to return uric acid and creatinine concentrations near to normal levels. Histopathological changes in kidney of diabetic rats were represented by condensed capillaries of glomeruli where the epithelia of these capillaries were hypertrophied and possessed pyknotic nuclei. Necrotic changes in proximal and distal convoluted tubules, loss of inner brush border lining proximal convoluted tubules, degeneration and infiltration of glomerular tufts by chronic inflammatory cells and red blood cells, swelling of tubular epithelium and enlargement of mesangial cells were also observed. On treatment with glibenclamide recovery was manifested in a general manner but limited parts of urineferous tubules designated earlier manifested diabetic changes of degeneration and cloudy swelling.

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